vector laboratories cat pi 2000 Search Results


anti rabbit hrp  (Vector Laboratories)
96
Vector Laboratories anti rabbit hrp
Anti Rabbit Hrp, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunoblotting with anti gfp  (TaKaRa)
95
TaKaRa immunoblotting with anti gfp
(A) Yeast cells co-expressing the indicated plasmids were grown on control (without Tryptophan and Leucine) or high stringency selection (without Tryptophan, Leucine, Histidine, and with 10 mM 3-AT) medium. ARID1 , full length cDNA of ARID1 ; ARID1-C , the C-terminus-containing ELM2 domain of ARID1; HDA8 , full length cDNA of HDA8 . AD is pGAD10. The cultures from each of the indicated strains were diluted 100-fold and spotted. Three colonies were streaked for each pair. (B) ARID1 interacts with HDA8 by GST pulldown assay. Whole cell extracts from wild type or <t>ARID1-GFP</t> plants were applied onto GST and GST-HDA8 (abbreviated GST-H8) beads. GFP and Hsc70 (loading control) antibodies were used in <t>immunoblotting.</t> (C) Stained protein gel showing proteins used for the GST pulldown assay. 1/30 of the amount of each protein used in was resolved by SDS-PAGE. GST-H8, full length HDA8 fused to GST; GST alone was used as a control. GST-H8 is marked with an arrowhead. (D) ARID1 interacts with HDA8 by Co-IP. Inflorescences from ARID1-Myc; HDA8-YFP (abbreviated H8-YFP) doubly transgenic plants or from wild type were immunoprecipitated with Myc antibody. Myc, GFP and Hsc70 (loading control) antibodies were used for immunoblotting.
Immunoblotting With Anti Gfp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated secondary antibody  (Vector Laboratories)
96
Vector Laboratories biotinylated secondary antibody
(A) Yeast cells co-expressing the indicated plasmids were grown on control (without Tryptophan and Leucine) or high stringency selection (without Tryptophan, Leucine, Histidine, and with 10 mM 3-AT) medium. ARID1 , full length cDNA of ARID1 ; ARID1-C , the C-terminus-containing ELM2 domain of ARID1; HDA8 , full length cDNA of HDA8 . AD is pGAD10. The cultures from each of the indicated strains were diluted 100-fold and spotted. Three colonies were streaked for each pair. (B) ARID1 interacts with HDA8 by GST pulldown assay. Whole cell extracts from wild type or <t>ARID1-GFP</t> plants were applied onto GST and GST-HDA8 (abbreviated GST-H8) beads. GFP and Hsc70 (loading control) antibodies were used in <t>immunoblotting.</t> (C) Stained protein gel showing proteins used for the GST pulldown assay. 1/30 of the amount of each protein used in was resolved by SDS-PAGE. GST-H8, full length HDA8 fused to GST; GST alone was used as a control. GST-H8 is marked with an arrowhead. (D) ARID1 interacts with HDA8 by Co-IP. Inflorescences from ARID1-Myc; HDA8-YFP (abbreviated H8-YFP) doubly transgenic plants or from wild type were immunoprecipitated with Myc antibody. Myc, GFP and Hsc70 (loading control) antibodies were used for immunoblotting.
Biotinylated Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated donkey anti goat polyclonal antibody  (Jackson Immuno)
93
Jackson Immuno biotinylated donkey anti goat polyclonal antibody
(A) Yeast cells co-expressing the indicated plasmids were grown on control (without Tryptophan and Leucine) or high stringency selection (without Tryptophan, Leucine, Histidine, and with 10 mM 3-AT) medium. ARID1 , full length cDNA of ARID1 ; ARID1-C , the C-terminus-containing ELM2 domain of ARID1; HDA8 , full length cDNA of HDA8 . AD is pGAD10. The cultures from each of the indicated strains were diluted 100-fold and spotted. Three colonies were streaked for each pair. (B) ARID1 interacts with HDA8 by GST pulldown assay. Whole cell extracts from wild type or <t>ARID1-GFP</t> plants were applied onto GST and GST-HDA8 (abbreviated GST-H8) beads. GFP and Hsc70 (loading control) antibodies were used in <t>immunoblotting.</t> (C) Stained protein gel showing proteins used for the GST pulldown assay. 1/30 of the amount of each protein used in was resolved by SDS-PAGE. GST-H8, full length HDA8 fused to GST; GST alone was used as a control. GST-H8 is marked with an arrowhead. (D) ARID1 interacts with HDA8 by Co-IP. Inflorescences from ARID1-Myc; HDA8-YFP (abbreviated H8-YFP) doubly transgenic plants or from wild type were immunoprecipitated with Myc antibody. Myc, GFP and Hsc70 (loading control) antibodies were used for immunoblotting.
Biotinylated Donkey Anti Goat Polyclonal Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse secondary antibody  (Vector Laboratories)
93
Vector Laboratories anti mouse secondary antibody
(A) Yeast cells co-expressing the indicated plasmids were grown on control (without Tryptophan and Leucine) or high stringency selection (without Tryptophan, Leucine, Histidine, and with 10 mM 3-AT) medium. ARID1 , full length cDNA of ARID1 ; ARID1-C , the C-terminus-containing ELM2 domain of ARID1; HDA8 , full length cDNA of HDA8 . AD is pGAD10. The cultures from each of the indicated strains were diluted 100-fold and spotted. Three colonies were streaked for each pair. (B) ARID1 interacts with HDA8 by GST pulldown assay. Whole cell extracts from wild type or <t>ARID1-GFP</t> plants were applied onto GST and GST-HDA8 (abbreviated GST-H8) beads. GFP and Hsc70 (loading control) antibodies were used in <t>immunoblotting.</t> (C) Stained protein gel showing proteins used for the GST pulldown assay. 1/30 of the amount of each protein used in was resolved by SDS-PAGE. GST-H8, full length HDA8 fused to GST; GST alone was used as a control. GST-H8 is marked with an arrowhead. (D) ARID1 interacts with HDA8 by Co-IP. Inflorescences from ARID1-Myc; HDA8-YFP (abbreviated H8-YFP) doubly transgenic plants or from wild type were immunoprecipitated with Myc antibody. Myc, GFP and Hsc70 (loading control) antibodies were used for immunoblotting.
Anti Mouse Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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30 diaminobenzidine dab vector laboratories cat sk 4100  (Vector Laboratories)
96
Vector Laboratories 30 diaminobenzidine dab vector laboratories cat sk 4100
(A) Yeast cells co-expressing the indicated plasmids were grown on control (without Tryptophan and Leucine) or high stringency selection (without Tryptophan, Leucine, Histidine, and with 10 mM 3-AT) medium. ARID1 , full length cDNA of ARID1 ; ARID1-C , the C-terminus-containing ELM2 domain of ARID1; HDA8 , full length cDNA of HDA8 . AD is pGAD10. The cultures from each of the indicated strains were diluted 100-fold and spotted. Three colonies were streaked for each pair. (B) ARID1 interacts with HDA8 by GST pulldown assay. Whole cell extracts from wild type or <t>ARID1-GFP</t> plants were applied onto GST and GST-HDA8 (abbreviated GST-H8) beads. GFP and Hsc70 (loading control) antibodies were used in <t>immunoblotting.</t> (C) Stained protein gel showing proteins used for the GST pulldown assay. 1/30 of the amount of each protein used in was resolved by SDS-PAGE. GST-H8, full length HDA8 fused to GST; GST alone was used as a control. GST-H8 is marked with an arrowhead. (D) ARID1 interacts with HDA8 by Co-IP. Inflorescences from ARID1-Myc; HDA8-YFP (abbreviated H8-YFP) doubly transgenic plants or from wild type were immunoprecipitated with Myc antibody. Myc, GFP and Hsc70 (loading control) antibodies were used for immunoblotting.
30 Diaminobenzidine Dab Vector Laboratories Cat Sk 4100, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse igg  (Vector Laboratories)
96
Vector Laboratories anti mouse igg
(A) Yeast cells co-expressing the indicated plasmids were grown on control (without Tryptophan and Leucine) or high stringency selection (without Tryptophan, Leucine, Histidine, and with 10 mM 3-AT) medium. ARID1 , full length cDNA of ARID1 ; ARID1-C , the C-terminus-containing ELM2 domain of ARID1; HDA8 , full length cDNA of HDA8 . AD is pGAD10. The cultures from each of the indicated strains were diluted 100-fold and spotted. Three colonies were streaked for each pair. (B) ARID1 interacts with HDA8 by GST pulldown assay. Whole cell extracts from wild type or <t>ARID1-GFP</t> plants were applied onto GST and GST-HDA8 (abbreviated GST-H8) beads. GFP and Hsc70 (loading control) antibodies were used in <t>immunoblotting.</t> (C) Stained protein gel showing proteins used for the GST pulldown assay. 1/30 of the amount of each protein used in was resolved by SDS-PAGE. GST-H8, full length HDA8 fused to GST; GST alone was used as a control. GST-H8 is marked with an arrowhead. (D) ARID1 interacts with HDA8 by Co-IP. Inflorescences from ARID1-Myc; HDA8-YFP (abbreviated H8-YFP) doubly transgenic plants or from wild type were immunoprecipitated with Myc antibody. Myc, GFP and Hsc70 (loading control) antibodies were used for immunoblotting.
Anti Mouse Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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texas red anti mouse igg  (Vector Laboratories)
93
Vector Laboratories texas red anti mouse igg
(A) Yeast cells co-expressing the indicated plasmids were grown on control (without Tryptophan and Leucine) or high stringency selection (without Tryptophan, Leucine, Histidine, and with 10 mM 3-AT) medium. ARID1 , full length cDNA of ARID1 ; ARID1-C , the C-terminus-containing ELM2 domain of ARID1; HDA8 , full length cDNA of HDA8 . AD is pGAD10. The cultures from each of the indicated strains were diluted 100-fold and spotted. Three colonies were streaked for each pair. (B) ARID1 interacts with HDA8 by GST pulldown assay. Whole cell extracts from wild type or <t>ARID1-GFP</t> plants were applied onto GST and GST-HDA8 (abbreviated GST-H8) beads. GFP and Hsc70 (loading control) antibodies were used in <t>immunoblotting.</t> (C) Stained protein gel showing proteins used for the GST pulldown assay. 1/30 of the amount of each protein used in was resolved by SDS-PAGE. GST-H8, full length HDA8 fused to GST; GST alone was used as a control. GST-H8 is marked with an arrowhead. (D) ARID1 interacts with HDA8 by Co-IP. Inflorescences from ARID1-Myc; HDA8-YFP (abbreviated H8-YFP) doubly transgenic plants or from wild type were immunoprecipitated with Myc antibody. Myc, GFP and Hsc70 (loading control) antibodies were used for immunoblotting.
Texas Red Anti Mouse Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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avidin d fitc conjugated  (Vector Laboratories)
96
Vector Laboratories avidin d fitc conjugated
(A) Yeast cells co-expressing the indicated plasmids were grown on control (without Tryptophan and Leucine) or high stringency selection (without Tryptophan, Leucine, Histidine, and with 10 mM 3-AT) medium. ARID1 , full length cDNA of ARID1 ; ARID1-C , the C-terminus-containing ELM2 domain of ARID1; HDA8 , full length cDNA of HDA8 . AD is pGAD10. The cultures from each of the indicated strains were diluted 100-fold and spotted. Three colonies were streaked for each pair. (B) ARID1 interacts with HDA8 by GST pulldown assay. Whole cell extracts from wild type or <t>ARID1-GFP</t> plants were applied onto GST and GST-HDA8 (abbreviated GST-H8) beads. GFP and Hsc70 (loading control) antibodies were used in <t>immunoblotting.</t> (C) Stained protein gel showing proteins used for the GST pulldown assay. 1/30 of the amount of each protein used in was resolved by SDS-PAGE. GST-H8, full length HDA8 fused to GST; GST alone was used as a control. GST-H8 is marked with an arrowhead. (D) ARID1 interacts with HDA8 by Co-IP. Inflorescences from ARID1-Myc; HDA8-YFP (abbreviated H8-YFP) doubly transgenic plants or from wild type were immunoprecipitated with Myc antibody. Myc, GFP and Hsc70 (loading control) antibodies were used for immunoblotting.
Avidin D Fitc Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated igg  (Vector Laboratories)
96
Vector Laboratories biotinylated igg
(A) Yeast cells co-expressing the indicated plasmids were grown on control (without Tryptophan and Leucine) or high stringency selection (without Tryptophan, Leucine, Histidine, and with 10 mM 3-AT) medium. ARID1 , full length cDNA of ARID1 ; ARID1-C , the C-terminus-containing ELM2 domain of ARID1; HDA8 , full length cDNA of HDA8 . AD is pGAD10. The cultures from each of the indicated strains were diluted 100-fold and spotted. Three colonies were streaked for each pair. (B) ARID1 interacts with HDA8 by GST pulldown assay. Whole cell extracts from wild type or <t>ARID1-GFP</t> plants were applied onto GST and GST-HDA8 (abbreviated GST-H8) beads. GFP and Hsc70 (loading control) antibodies were used in <t>immunoblotting.</t> (C) Stained protein gel showing proteins used for the GST pulldown assay. 1/30 of the amount of each protein used in was resolved by SDS-PAGE. GST-H8, full length HDA8 fused to GST; GST alone was used as a control. GST-H8 is marked with an arrowhead. (D) ARID1 interacts with HDA8 by Co-IP. Inflorescences from ARID1-Myc; HDA8-YFP (abbreviated H8-YFP) doubly transgenic plants or from wild type were immunoprecipitated with Myc antibody. Myc, GFP and Hsc70 (loading control) antibodies were used for immunoblotting.
Biotinylated Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse igg secondary antibody  (Vector Laboratories)
96
Vector Laboratories anti mouse igg secondary antibody
(A) Yeast cells co-expressing the indicated plasmids were grown on control (without Tryptophan and Leucine) or high stringency selection (without Tryptophan, Leucine, Histidine, and with 10 mM 3-AT) medium. ARID1 , full length cDNA of ARID1 ; ARID1-C , the C-terminus-containing ELM2 domain of ARID1; HDA8 , full length cDNA of HDA8 . AD is pGAD10. The cultures from each of the indicated strains were diluted 100-fold and spotted. Three colonies were streaked for each pair. (B) ARID1 interacts with HDA8 by GST pulldown assay. Whole cell extracts from wild type or <t>ARID1-GFP</t> plants were applied onto GST and GST-HDA8 (abbreviated GST-H8) beads. GFP and Hsc70 (loading control) antibodies were used in <t>immunoblotting.</t> (C) Stained protein gel showing proteins used for the GST pulldown assay. 1/30 of the amount of each protein used in was resolved by SDS-PAGE. GST-H8, full length HDA8 fused to GST; GST alone was used as a control. GST-H8 is marked with an arrowhead. (D) ARID1 interacts with HDA8 by Co-IP. Inflorescences from ARID1-Myc; HDA8-YFP (abbreviated H8-YFP) doubly transgenic plants or from wild type were immunoprecipitated with Myc antibody. Myc, GFP and Hsc70 (loading control) antibodies were used for immunoblotting.
Anti Mouse Igg Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2000 rrid ab 2336617  (Vector Laboratories)
99
Vector Laboratories 2000 rrid ab 2336617

2000 Rrid Ab 2336617, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Yeast cells co-expressing the indicated plasmids were grown on control (without Tryptophan and Leucine) or high stringency selection (without Tryptophan, Leucine, Histidine, and with 10 mM 3-AT) medium. ARID1 , full length cDNA of ARID1 ; ARID1-C , the C-terminus-containing ELM2 domain of ARID1; HDA8 , full length cDNA of HDA8 . AD is pGAD10. The cultures from each of the indicated strains were diluted 100-fold and spotted. Three colonies were streaked for each pair. (B) ARID1 interacts with HDA8 by GST pulldown assay. Whole cell extracts from wild type or ARID1-GFP plants were applied onto GST and GST-HDA8 (abbreviated GST-H8) beads. GFP and Hsc70 (loading control) antibodies were used in immunoblotting. (C) Stained protein gel showing proteins used for the GST pulldown assay. 1/30 of the amount of each protein used in was resolved by SDS-PAGE. GST-H8, full length HDA8 fused to GST; GST alone was used as a control. GST-H8 is marked with an arrowhead. (D) ARID1 interacts with HDA8 by Co-IP. Inflorescences from ARID1-Myc; HDA8-YFP (abbreviated H8-YFP) doubly transgenic plants or from wild type were immunoprecipitated with Myc antibody. Myc, GFP and Hsc70 (loading control) antibodies were used for immunoblotting.

Journal: PLoS Genetics

Article Title: An ARID Domain-Containing Protein within Nuclear Bodies Is Required for Sperm Cell Formation in Arabidopsis thaliana

doi: 10.1371/journal.pgen.1004421

Figure Lengend Snippet: (A) Yeast cells co-expressing the indicated plasmids were grown on control (without Tryptophan and Leucine) or high stringency selection (without Tryptophan, Leucine, Histidine, and with 10 mM 3-AT) medium. ARID1 , full length cDNA of ARID1 ; ARID1-C , the C-terminus-containing ELM2 domain of ARID1; HDA8 , full length cDNA of HDA8 . AD is pGAD10. The cultures from each of the indicated strains were diluted 100-fold and spotted. Three colonies were streaked for each pair. (B) ARID1 interacts with HDA8 by GST pulldown assay. Whole cell extracts from wild type or ARID1-GFP plants were applied onto GST and GST-HDA8 (abbreviated GST-H8) beads. GFP and Hsc70 (loading control) antibodies were used in immunoblotting. (C) Stained protein gel showing proteins used for the GST pulldown assay. 1/30 of the amount of each protein used in was resolved by SDS-PAGE. GST-H8, full length HDA8 fused to GST; GST alone was used as a control. GST-H8 is marked with an arrowhead. (D) ARID1 interacts with HDA8 by Co-IP. Inflorescences from ARID1-Myc; HDA8-YFP (abbreviated H8-YFP) doubly transgenic plants or from wild type were immunoprecipitated with Myc antibody. Myc, GFP and Hsc70 (loading control) antibodies were used for immunoblotting.

Article Snippet: The bound (pellet) and unbound (supernatant) proteins were detected by immunoblotting with anti-GFP (Cat.632480, Clontech, 1∶2000 dilution) and anti-Hsc70 (Cat.SPA-818, Stressgen, 1∶10000 dilution) antibodies.

Techniques: Expressing, Selection, GST Pulldown Assay, Western Blot, Staining, SDS Page, Co-Immunoprecipitation Assay, Transgenic Assay, Immunoprecipitation

Journal: Cell reports

Article Title: Integrating Gene and Protein Expression Reveals Perturbed Functional Networks in Alzheimer’s Disease

doi: 10.1016/j.celrep.2019.06.073

Figure Lengend Snippet:

Article Snippet: Normal Horse Serum , Vector Laboratories , Cat# S-2000; RRID: AB_2336617.

Techniques: Plasmid Preparation, Recombinant, Software, Microscopy